Reevaluation of Candida susceptibility to amphotericin B: comparative study using isolates from three hospitals in the state of Rio Grande do Sul

Revista da Sociedade Brasileira de Medicina Tropical

Dal Forno, Nara Frasson¹  Lopes, Paulo Guilherme Markus¹  Hospital Universitário de Santa Maria, Santa Maria¹  Spader, Tatiana¹  Universidade Federal do Rio Grande do Sul, Porto Alegre¹  Aquino, Valério¹  Severo, Luiz Carlos¹  Hospital de Clínicas de Porto Alegre, Porto Alegre¹  Santurio, Janio Morais¹  Loreto, Érico¹  Scheid, Liliane Alves¹  Alves, Sydney Hartz¹  Universidade Federal de Santa Maria, Santa Maria¹  Boff, Everton¹ 

关键词: Susceptibility;    Amphotericin B;    Candida;    Candidemia;   

DOI  :  10.1590/S0037-86822008000100007

学科分类：农业科学（综合）

来源: Sociedade Brasileira de Medicina Tropical

【 摘 要 】

Susceptibility to amphotericin B was compared between isolates of Candida spp that were obtained from candidemia cases as follows: 41 from Hospital Universitário de Santa Maria, 56 from Hospital de Clínicas, Porto Alegre, and 47 from the Santa Casa hospital complex, Porto Alegre. The tests were based on the document M27-A2 from the Clinical Laboratory Standards Institute, but with 20 concentrations of amphotericin B ranging from 0.1 to 2mg/ml. The tests were carried out using RPMI 1640 medium with glucose, antibiotic medium 3 and yeast nitrogen base-dextrose. The antibiotic medium 3 broth generated wide ranges of minimum inhibitory concentrations and minimum fungicidal concentrations in relation to the other agents. The variations in susceptibility between the hospitals were best detected in antibiotic medium 3. The isolates from Hospital Universitário de Santa Maria showed lower susceptibility than did those from the Santa Casa hospital complex, Porto Alegre (p < 0.05). The causes of the susceptibility variations were not assessed but they indicate the need for surveillance regarding the susceptibility to amphotericin B.

【 授权许可】

Unknown