Cloning and expression of the transmembranic glycoprotein from human T cell lymphotropic virus in a prokaryotic system

Revista da Sociedade Brasileira de Medicina Tropical

Universidade de São Paulo, Ribeirão Preto¹  Kashima, Simone¹  Russo-Carbolante, Elisa Maria de Sousa¹  Covas, Dimas Tadeu¹  Penteado, Flora Cristina Lobo¹  Medeiros, Luciene¹  Takayanagui, Oswaldo M.¹  Universidade Estadual Paulista, Araraquara¹ 

关键词: gp21;    HTLV-1;    Recombinant proteins;    Heterologous expression;   

DOI  :  10.1590/S0037-86822007000300005

学科分类：农业科学（综合）

来源: Sociedade Brasileira de Medicina Tropical

【 摘 要 】

HTLV-1 is the virus that causes T cell lymphoma/leukemia in adults and a neurological disorder known as HTLV-associated myelopathy or tropical spastic paraparesis. One of the transmission means is through contaminated blood and its byproducts. Because of the risk of HTLV-associated infections, screening for HTLV was introduced for Brazilian blood donors in 1993. Most of the diagnostic kits used in the national blood banks are bought from foreign companies. Brazil does not have the technology to produce this material and there is a need to produce diagnostic systems with national technology. In this study, we show the expression of gp21/HTLV-1 in Escherichia coli and its reactivity towards monoclonal antibodies and the antibodies of infected patients. Expressing these proteins is the first step towards obtaining diagnostic kits with Brazilian biotechnology.

【 授权许可】

Unknown