Enzyme-linked Immunosorbent Assay biotin/avidin method standardization, for identification of Lutzomyia (Lutzomyia) longipalpis bloodmeals (Lutz & Neiva, 1912)

Revista da Sociedade Brasileira de Medicina Tropical

Consales, Cleide Aschenbrenner¹  Instituto Pasteur, São Paulo¹  Galati, Eunice Aparecida Bianchi¹  Instituto Adolfo Lutz, São Paulo¹  Marassá, Ana Maria¹  Universidade de São Paulo, São Paulo¹ 

关键词: Blood meal identification;    Lutzomyia longipalpis;    Phlebotominae;    Sandwich enzyme-linked immunosorbent assay;    Vectors;   

DOI  :  10.1590/S0037-86822004000600003

学科分类：农业科学（综合）

来源: Sociedade Brasileira de Medicina Tropical

【 摘 要 】

Bloodmeals taken by insects constitute an important parameter for clarifying aspects of the transmission of zoonoses, including leishmaniases. Immunological assays can be used to investigate the attraction of vectors to animals, which may be hosts of these parasitoses. The objective of this study was to standardize a sandwich enzyme-linked immunosorbent assay and titer samples with different time periods of digestion, in laboratory-bred Lutzomyia longipalpis fed on rats. In the light of the high sensitivity that the biotin-avidin method permits, the technique provided at least ninety repeat tests for each sample and identified recent bloodmeals taken by these insects. Bloodmeals were detectable up to 12 and 24h after blood ingestion, and a significant difference between these titers was observed.

【 授权许可】

Unknown