Aminoquinolone WR6026 as a feasible substitute for gentian violet in Chagas' disease prophylaxis in preserved blood for transfusional purposes

Revista da Sociedade Brasileira de Medicina Tropical

Universidade de Uberaba, Uberaba¹  Universidade Federal de Minas Gerais, Belo Horizonte¹  Faculdade de Medicina do Triângulo Mineiro, Uberaba¹  Barretto, Orlando César de Oliveira Pereira¹  Nonoyama, Kimyo¹  Grolg, M.¹  Hemocentro Regional de Uberaba, Uberaba¹  Instituto Adolfo Lutz, São Paulo¹  Pianetti, Gisele Marilia¹  Moraes-Souza, Helio¹  Walter Reed Army Institute of Research, Silver Spring, USA¹  Chiari, Egler¹  Universidade de São Paulo, São Paulo¹ 

关键词: Transfusion-associated Chagas' disease;    Chemoprophylaxis;    Aminoquinolone;    Blood transfusion;   

DOI  :  10.1590/S0037-86822002000600003

学科分类：农业科学（综合）

来源: Sociedade Brasileira de Medicina Tropical

【 摘 要 】

The search for a colorless, nontoxic and efficient drug to prevent transfusion-associated Chagas' disease (TACD) has been underway unsuccessfully since 1953 when gentian violet was preconized and to date is still being used as the only in vitro trypanocidal agent. The recent findings of aminoquinolone "WR6026" as a trypanocidal agent, led the authors to study the metabolism of red cells stored with this compound, the main objective of which was to define its applicability in TACD control. Ten units of human whole blood collected in CPDA-1 were divided into two equal satellite bags. One had "WR6026" (final concentration 62.5µg/mL) added and the other was used as a control, both were stored at 4oC. At baseline, day 7, 14, 21 and 28, samples were taken for the following measurements: adenosine triphosphate (ATP), hemoglobin, electrolytes (sodium and potassium), gases (pO2 and pCO2) and osmotic fragility. The results of tests and control were analyzed through parametric t-student test. The results were similar in both groups throughout the experiment except for the level of ATP on day 14, which presented significantly higher values in the tests when compared with the controls (p = 0.012). It was concluded that WR6026 does not interfere in the preservation and probably the viability of the erythrocytes also until day 28 of storage. Consequently the authors suggest that WR6026 could emerge as a colorless substitute for gentian violet in the control of TACD in endemic areas.

【 授权许可】

Unknown