The value of in vitro cell culture of granulocytes in the detection of Ehrlichia

Revista da Sociedade Brasileira de Medicina Tropical

Mutani, Alex¹  The University of The West Indies, Saint Augustine, Trinidad and Tobago¹  Kaminjolo, James Stwart¹ 

关键词: Ehrlichia;    Cell Culture;    Mononuclear cells;   

DOI  :  10.1590/S0037-86822001000400012

学科分类：农业科学（综合）

来源: Sociedade Brasileira de Medicina Tropical

【 摘 要 】

Peripheral blood leukocytes from different animals were isolated from whole blood and maintained in Dulbeco's medium containing homologous serum without antibiotics. After 72 hrs microscopic examination of these cells showed that most animals were infected with Ehrlichia. Observation of thin blood smears from the same animals showed that only two were positive for Ehrlichia. The results of this investigation show that leukocyte culture is superior to the traditional thin blood film method in the detection of Ehrlichia and that asymptomatic carriers are easily detected. The method is inexpensive and does not require specific cell lines although it is necessary to use sterile sera.

【 授权许可】

Unknown