Utilização do picro-sirius para corar o Paracoccidioides brasiliensis

Revista da Sociedade Brasileira de Medicina Tropical

Silveira, Suzana Aperecida¹  Reis, Marlene Antonia dos¹  Almeida, Hipólito de Oliveira¹  Morais, Maria das Graças Reis de¹  Brandão, Marilda da Costa¹ 

关键词: Paracoccidioides brasiliensis;    Paracoccidiodomycosis;    Picrosirius;    Histotechnology;    Mycosis;   

DOI  :  10.1590/S0037-86821988000200005

学科分类：农业科学（综合）

来源: Sociedade Brasileira de Medicina Tropical

【 摘 要 】

Picrosirius stain was used for Paracoccidioides brasiliensis in formalin-fixed human tissue. By common light microscopy the fungus shows a red peripheral band and on polarization microscopy, one or more birefringent green rings. Previous immersion in 1% sodium hidroxide solution enhances the staining and the birefringency of parasitic cells. The differentiation of the stained sections with 0,5% sodium hydroxide eliminates the birefringency of the collagen more rapidly than that of the paraeoccidioides.

【 授权许可】

Unknown