Teste reverso para anticorpos igm na toxoplasmose, com hemácias sensibilizadas por antígenos de Toxoplasma gondii

Revista da Sociedade Brasileira de Medicina Tropical

Camargo, Mário E.¹  Almeida, Gastão de¹  Navarrete, Nancy¹  Instituto de Medicina Tropical de São Paulo, São Paulo, Brasil¹  Universidade Austral de Chile¹ 

关键词: Toxoplasmosis;    Immunodiagnosis;    IgM;   

DOI  :  10.1590/S0037-86821984000100003

学科分类：农业科学（综合）

来源: Sociedade Brasileira de Medicina Tropical

【 摘 要 】

A test is described for the detection oflgM anti-toxoplasma antibodies based on the technique of serum IgM capture by anti-IgM antibodies adsorbed to plasticplates. A suspension of formolized human erythrocytes, sensitized with Toxoplasma gondii antigens is then used as a indicative of anti-toxoplasma specificity of the captured antibodies. The use of sensitized red-cells instead of parasite suspensions, as previously described by Desmonts et al., 1981, results in a more practical test. Adequate red cells suspensions are easily prepared just by diluting the usual toxoplasmosis hemagglutination reagent and reading the test is much facilitated with red cells as indicator than with the colorlessparasites, with clear- cut results.The test was comparable in sensitivity to the IgM-immunofluorescence test, but with no inteiference of rheumatoid factor or competition oflgG antibodies.

【 授权许可】

Unknown