Zeta Chain-Associated Protein-70 Expression is a Prognostic Tool in Mature and Precursor B-Cell Neoplasm | Science Publications

American Medical Journal

Rabab M. Mahfoz¹  Sherin A.A. ElAziz¹  Heba A.E. Deghadi¹  Abd E.-H.A. Metwali¹  Nadia E. ElMenshawy¹ 

关键词: ZAP-70;    cell neoplasm;    Zeta Chain-Associated Protein (ZAP);    T-Cell Receptor (TCR);    therapeutic target;    binnet staging;    powerful prognostic;    positive zap;    bechman coulter;   

DOI  :  10.3844/amjsp.2011.40.46

学科分类：基础医学

来源: Science Publications

【 摘 要 】

Problem statement: The Zeta Chain-Associated Protein (ZAP) is a 70-kDa moleculeassociated with the zeta chain of the CD3 receptor complex of the lymphocyte population. It plays animportant role in signaling initiation, activation T-cell receptor, phosphorylation of multipledownstream targets. So that believed as T. lymphocyte specific .Several studies is gathering that thepattern of expression is not lineage specific. We aimed to study the role of ZAP-70 /CD38 as newprognostic marker in CLL and its prognostic value in ALL patients. Approach: Our study included 49ALL ranged from 2-42 years they were 27 males and 22 females. One hundred fifty six patients withCLL. They were 87 males and 69 females, their ages ranged from 50-77 yearsImmunophenotyping. byflow cytometry (EPICS XL-2and 4 color Bechman Coulter USA), intracellular ZAP70 protein wasassessed by flow cytometry using (Caltag laboratories, Beckman Coulter USA). Results:Immunophenotyping of 49 ALL patients ZAP-70 was found at significantly higher levels in T-ALLcases compared with B-lineage (p<0.001), however, a proportion of B- lineage ALL showed highlevels of ZAP-70. Moreover we observed that ZAP-70 is higher in pre-B ALL than pro B ALL andcommon ALL with more decline in mature B-ALL. Follow up patient by zap, pretreatment versus posttreatment p = 0.008, correlation of zap with hematological and clinical parameter in ALL, no significantcorrelation except positively correlated with leucocytic count while in CLL highly significant correlationwith splenomegally but not with lymphadenopathy, strong association between zap-70 and binnet stagingbut not with CD38. Overall survival of zap-70 in ALL/CLL show that short free survival associated withpositive zap-70. ZAP-70 has raised great interest in CLL patients because it represents a powerfulprognostic marker. Conclusion: Our results provide preliminary indication on the potential use of thisprotein as a prognostic marker also in ALL. These results clearly show that ZAP-70 may indeed be atherapeutic target. ZAP-70 expression in a subset of patients with ALL opens the perspective ofinvestigating the use of an inhibitor and can be a candidate molecule for targeted therapy.

【 授权许可】

Unknown