Thrombophilic Genes Mutations in Women with Repeated In-Vitro Fertilization Failure | Science Publications

American Medical Journal

Ahmad Y. Rezk¹  Naglaa Fathy Al Husseini¹  Amal I. Ali¹  Mosad M. Odah¹  Shaymaa M.A. El Rahman¹ 

关键词: Recurrent Pregnancy Loss (RPL);    Thrombophilia-mutation-IVF-multiplex PCR-Reverse hybridization;    Methylenetetrahydrofolate Reductase (MTHFR);    Nitro Blue Tetrazolium (NBT);    In-Vitro Fertilization (IVF);    Intracytoplasmic Sperm Injection (ICSI);    Prothrombin (PT);    Body Mass Index (BMI);    Factor V Ledien (FVL);   

DOI  :  10.3844/amjsp.2011.7.12

学科分类：基础医学

来源: Science Publications

【 摘 要 】

Problem statement: Thrombophilia has been recently implicated in early pregnancy lossand IVF implantation failure, by impairing the initial vascularization process occurring at implantation,which is necessary for a successful pregnancy. Approach: The aim of this study is to assess thepresence possibility of mutation in thrombophilic genes [Factor V (FV) gene, Prothrombin (PT) geneand Methylenetetrahydrofolate Reductase (MTHFR) gene] in women with repeated IVF-embryotransfer failure with unknown causes. The study was performed on patients with two or morepreviously failed IVF-embryo transfer cycles with unknown causes. Women who conceivedspontaneously with no previous history of miscarriage and women who have had successful pregnancyafter their first IVF-embryo transfer cycle are included as control groups. Results: There are increasein allelic frequencies of thrombophilic genes (factor V, prothrombin and methylenetetrahydrofolatereductase) mutation, in addition to genotype frequencies (both homozygote and heterozygote) andincrease in frequency of multiple gene mutations among women with IVF- embryo transfer failure.Conclusion: Thrombophilia has a significant role in IVF- embryo transfer implantation failure.Women with repeated IVF- embryo transfer failure should be screened for Thrombophilia to avoidrepeated IVF- embryo transfer failure

【 授权许可】

Unknown