Androgen Receptor in Macrophages of Male Rat is Greater Than in Female | Science Publications

American Journal of Immunology

Kazem Ahmadi¹  Alan B. McCruden¹ 

关键词: Macrophage androgen receptor;    immunomagnetic;    male and female;   

DOI  :  10.3844/ajisp.2005.48.54

学科分类：过敏症与临床免疫学

来源: Science Publications

【 摘 要 】

he presence and possible sex differences of androgen receptor in peritoneal macrophages was investigated using immunomagnetic beads. Macrophages were incubated with different concentrations of [³H]-5αDHT in the presence or absence of a 100 fold excess of unlabelled 5α-DHT. Labelled cells were separated from unbound steroid by immunomagnetic beads coated with anti-rat macrophage antibody. The binding identified in the rat macrophages was highly selective towards androgenic compounds. The dissociation constant (kd) value for the receptor was calculated to be 3.3X10^-9M and 5X10^-9M for macrophages of male and female rat respectively. The number of receptors in each cell was 792

【 授权许可】

Unknown