Distribution of Enterotoxigenic Escherichia coli among E. coli isolates from diarrheal samples referred to educational hospitals in Tehran-Iran

Novelty in Biomedicine

Hossein Goudarzi²  Mohammad Mohammadzadeh¹  Hossein Dabiri²  Fatemeh Fallah² 

[1] Microbiology department, Shahid Beheshti university of medical science, School of medicine.;Department of Medical Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran

关键词: Enterotoxigenic Escherichia coli;    Diarrhea;    lt;    st.;   

DOI  :  

学科分类：生物科学（综合）

来源: Shahid Beheshti University of Medical Sciences

【 摘 要 】

Background: Introduction: Enterotoxigenic Escherichia coli (ETEC) is the most important bacterial cause of watery  travelers' diarrhea  in  developing countries. Watery diarrhea is can cause serious life-threatening dehydration. ETEC was caused diarrhea by the secretion of two heat-labile enterotoxins (LTs) and the heat-stable enterotoxins (STs) which increase intestinal secretion. Routine laboratory methods are not appropriate to detect ETEC and other diarrheagenic E. coli pathotypes. The molecular techniques such as PCR are rapid and accurate methods that have been developed for detection of ETEC. We were recognized ETEC by PCR on lt and st genes from E. coli isolates from patients with diarrhea collected from selected Tehran educational hospitals. Materials and Methods:TheE. coliisolates were collected from total 140 patients with diarrhea and 110 patients without diarrhea using culture and IMViC test. DNA was extracted by boiling method and the presence of the uidA, lt and st genes was detected by PCR. Results:Among 140E. coliisolates from diarrheal stools 5 (3.6%) isolates were positive for, justltgene, 3 (2.1%) co-amplified for bothlt / stand 1 (0.7%) was positive for just thestgene which were considered as ETEC. In theE. coliisolates from non-diarrheal control samples just one (0.9%) isolate was positive for bothltandstgenes. Conclusion:The results showed that the ETEC as a significant cause of diarrhea, usually ignored by laboratories using traditional methods. Sometimes the ETEC causes severe diarrhea and can threaten for patient's life. Thus a rapid diagnostic test such as PCR can be very helpful in the treatment of patients.

【 授权许可】

Unknown