Detection of actA and InlB genes in Listeria monocytogenes Isolated from women with Spontaneous abortions

Novelty in Biomedicine

Robabe Taherpanah²  Arezo Taherpor³  Neda Baseri¹  Gita Eslami¹  Roghayeh Samadi¹ 

[1] Department of Microbiology, Faculty of Medicine, Shahid Beheshti University, Medical Sciences, Tehran, Iran.;Infertility and Reproductive Health Research Center, Faculty of medicine , Shahid Beheshti University, Medical Sciences, Tehran, Iran.;Department of Microbiology, Faculty of Medicine,Kordestan University, Medical Sciences, Kordestan, Iran.

关键词: RLDH;    Discectomy;    PLIF;   

DOI  :  

学科分类：生物科学（综合）

来源: Shahid Beheshti University of Medical Sciences

【 摘 要 】

  AbstractBackground & ObjectiveTo assess the extent of L. monocytogenes in causation of human spontaneous abortions by isolation methods and PCR analysis for the presence of actA and InlB genes. Methods: In this descriptive study, vaginal swabs were collected from 96 women with spontaneous abortions who referred to Tehran`s hospitals. The samples were cultured in to Listeria specific media (PALCAM agar). Then , the Listeria genus was verified by differentiation  biochemical tests, such as, haemolysis on Blood agar, Catalase and Oxidase reaction, motility at room temperature. PCR test was performed on all samples and detected the actA and InlB genes of L. monocytogenes. Results: In culture, 7 of 96 samples were positive for L.monocytogenes.                                     in PCR technique, actA and InlB genes were detected from 12 and 2 vaginal samples,  respectively. Conclusions: The occurrence of pathogenic L. monocytogenes in cases of spontaneous abortions was 12.5%. It seems that the actA and InlB genes have an important roles as essential virulences determinant in pathogenic L.monocytogenes. The results show the PCR method is more sensitive, easier and faster than culture to detect L.monocytogenes. Key word: Listeria monocytogenes , spontaneous abortions , actA gene , InlB gene

【 授权许可】

Unknown