Effect of temperature, concentration and contact time of sodium hypochlorite on the treatment and revitalization of oral biofilms

Journal of Dental Research, Dental Clinics, Dental Prospects

Clovis Monteiro Bramante¹  Aldo del Carpio-Perochena²  Bruno Cavalini Cavenago¹  Samuel Lucas Fernandes¹  Flaviana Bombarda de Andrade¹  Marco Hungaro Duarte¹  Marcia Zardin Graeff¹  Marina Marciano da Silva¹ 

[1] Bauru Dental School, University of Sao Paulo

关键词: Bacteria;    biofilms;    dentin;    sodium hypochlorite;    temperature;   

DOI  :  

学科分类：农业科学（综合）

来源: Tabriz University of Medical Sciences * Faculty of Dentistry

【 摘 要 】

Background and aims. Increasing the temperature of sodium hypochlorite (NaOCl) enhances its dissolution and antibacterial properties. However, the high resistance of multi-species biofilms could restrict the effect of the solution regardless of its temperature, enabling the long-term recovery of the surviving bacteria. The aim of this study was to investigate if the increase of temperature of NaOCl improves its antibacterial and dissolution ability on oral biofilms and if the post-treatment remaining bacteria were capable of growing in a nutrient-rich medium.Materials and methods. Forty dentin blocks were infected intra-orally for 48 hours. Then, the specimens were treated with 1% and 2.5% NaOCl at room temperature (22ºC) and body temperature (37ºC) for 5 and 20 min. The percentage of live cells and the biovolume were measured pre- (control) and post-treatment and after the biofilm revitalization. Four confocal ‘stacks’ were chosen from random areas of each sample. Statistical analysis was performed using Kruskal-Wallis and Dunn tests. Statistical significance was defined at P0.05). The 1%-NaOCl for 5min was not able to significantly kill the bacteria, regardless of its temperature and contact time (P >0.05).Conclusion. The temperature variation of the NaOCl was not relevant in killing or dissolving bacterial biofilms. Twenty-four hours of reactivation did not appear to be enough time to induce a significant bacterial growth.

【 授权许可】

Unknown