Cissampelos sympodialis has anti-viral effect inhibiting dengue non-structural viral protein-1 and pro-inflammatory mediators

Revista Brasileira de Farmacognosia

Barbosa Filho, José Maria¹  Marinho, Cintia Ferreira¹  Piuvezam, Marcia Regina¹  Kubelka, Claire Fernandes¹  Lima, Ana Luisa de Araujo¹  Mello, Cíntia da Silva¹  Faculdade São Francisco da Paraíba, Cajazeiras, Brazil¹  Fialho, Luciana Gomez¹  Fundação Oswaldo Cruz, Rio de Janeiro, Brazil¹  Leite, Fagner Carvalho¹  Universidade Federal da Paraíba, João Pessoa, Brazil¹ 

关键词:  Cissampelos sympodialis;    Dengue;    Huh-7 cells;    Cytokines;    NS1;    Warifteine;   

DOI  :  10.1016/j.bjp.2016.03.013

来源: Sociedade Brasileira de Farmacognosia

【 摘 要 】

Dengue is the most important viral infection transmitted among humans by arthropod-borne. There are currently no vaccines or specific therapeutical treatment. Therefore, immunomodulatory compounds from plants have been widely examined for their antiviral effects. Cissampelos sympodialis Eichler, Menispermaceae, has scientifically proven to present immunomodulatory activities. Here we assessed the antiviral activity of leaf hydroalcoholic extract, warifteine or methylwarifteine from C. sympodialis in an in vitro dengue virus infection model. The results demonstrated that leaf hydroalcoholic extract or warifteine/methylwarifteine treatment did not reduce dengue virus-Ag+ hepatocyte (Huh-7 cell) rates in present experimental conditions. However, we assessed the potential antiviral effect of leaf hydroalcoholic extract or warifteine/methylwarifteine on dengue virus-infection by the production of inflammatory molecules, TNF-α, MIF, IL-8 and PGE2. Dengue virus infection enhanced TNF-α, MIF, IL-8 and PGE2 production in infected Huh-7 cells and leaf hydroalcoholic extract but not warifteine/methylwarifteine treatments, significantly reduced these molecules in infected cells. In dengue virus-infected Huh-7 cells, non-structural protein-1 is produced and leaf hydroalcoholic extract significantly inhibited it independently of alkaloids. Our findings imply that leaf hydroalcoholic extract may attenuate dengue virus infection in Huh-7 cells by inhibiting the enhanced of pro-inflammatory mediators and non-structural protein-1 production induce by dengue virus independently of warifteine/methywarifteine its major compound.

【 授权许可】

Unknown