期刊论文详细信息
Revista Brasileira de Farmacognosia
In vitro and in vivo evaluation of efficacy and safety of photoprotective formulations containing antioxidant extracts
Leitão, Álvaro A.C.1  Cerqueira-Coutinho, Cristal1  Silva, Ronald S.1  Vermelho, Alane Beatriz1  Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil1  Mansur, Maria Cristina P.P. Reis1  Ricci-Júnior, Eduardo1  Leitão, Gilda G.1  Leitão, Suzana Guimarães1  Presgrave, Octávio A.F.1  Santos, Elisabete P.1  Fundação Oswaldo Cruz, Rio de Janeiro, Brazil1 
关键词:  Leaf extracts;    Cosmetic formulation;    Photoprotection;    Efficacy and safety;    Evaluation methods;   
DOI  :  10.1016/j.bjp.2015.11.006
来源: Sociedade Brasileira de Farmacognosia
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【 摘 要 】

Chronic exposure to solar radiation could contribute to premature skin aging and skin cancer. Skin presents its own antioxidant defense, however when defenses are out of balance, reactive oxygen species could damage biological structures. In the present work, an oil-in-water photoprotective emulsion was developed and Bauhinia microstachya var. massambabensis Vaz, Fabaceae, extracts at 1% (obtained by extraction with different solvents) were added to this emulsion. In vitro and in vivo efficacy and safety of the formulations were evaluated. Spectrophotometric methods and in vivo Colipa test were performed to evaluated efficacy of the formulations, through sun protection factor (SPF) determination and UVA protection factor assessment. To the in vitro safety assessment HET-CAM, CAM-TBS and Red Blood Cell tests were performed. Results showed that both extracts contributed to a higher in vivo photoprotection (SPF 18) when compared to the formulation without extract (SPF 13), this result could be attributed to the antioxidant activity of the plant extracts that act by capturing reactive oxygen species. Concerning safety, all formulations were considered non-irritant according to in vitro tests. Formulations containing extracts could be considered efficient and safe for cosmetic use since they presented higher sun protection factor and passed the toxicity tests.

【 授权许可】

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