Evaluation of the orofacial antinociceptive profile of the ethyl acetate fraction and its major constituent, rosmarinic acid, from the leaves of Hyptis pectinata on rodents

Revista Brasileira de Farmacognosia

Universidade Federal de Sergipe, São Cristóvão, Brazil¹  Silva, Tania M.S.¹  Universidade Federal Rural de Pernambuco, Recife, Brazil¹  Lima, Adley A.N.¹  Correia, Maria Tereza S.¹  Falcão, Rosângela E.A.¹  Universidade Federal Pernambuco, Recife, Brazil¹  Camara, Celso A.¹  Universidade Federal Sergipe, Lagarto, Brazil¹  Quintans, Jullyana S.S.¹  Universidade Federal do Rio Grande do Norte, Natal, Brazil¹  Guimarães, Adriana G.¹  Quintans-Júnior, Lucindo J.¹  Santos, Priscila L.¹  Souza, Silvana A. de¹ 

关键词:  Hyptis pectinate;    Rosmarinic acid;    Orofacial pain;    Nociception;   

DOI  :  10.1016/j.bjp.2015.07.029

来源: Sociedade Brasileira de Farmacognosia

【 摘 要 】

Hyptis pectinata (L.) Poit., Lamiaceae, popularly known as "sambacaitá," is an aromatic shrub largely grown in the Brazilian northeastern. We investigated the antinociceptive effects of the ethyl acetate fraction obtained from the leaves of H. pectinata and of its main constituent rosmarinic acid, on formalin (2%)-, glutamate (25 µM)- and capsaicin (2.5 µg)-induced orofacial nociception in rodents. Male mice were pretreated with ethyl acetate fraction (100, 200 or 400 mg/kg, p.o.), rosmarinic acid (10 or 20 mg/kg, p.o.), morphine (5 mg/kg, i.p.), or vehicle (distilled water + 0.2% Tween 80). Ethyl acetate fraction reduced the nociceptive face-rubbing behavior during the two phase of the formalin test, whereas pretreatment with rosmarinic acid decreased the pain behavior in the second phase. Ethyl acetate fraction produced significant antinociceptive effects in the capsaicin and glutamate tests. This study showed that oral administration of ethyl acetate fraction produced potent antinociceptive effects compared to treatment with rosmarinic acid.

【 授权许可】

Unknown