Antimicrobial (including antimollicutes), antioxidant and anticholinesterase activities of Brazilian and Spanish marine organisms – evaluation of extracts and pure compounds

Revista Brasileira de Farmacognosia

Boeder, Ariela Maína¹  Santos, Larissa dos¹  Fundação Universidade Regional de Blumenau, Blumenau, Brazil¹  Zimath, Priscila Laiz¹  Kuroshima, Kátia Naomi¹  Universidade do Vale do Itajaí, Itajaí, Brazil¹  Cordova, Caio Maurício Mendes de¹  Rebelo, Ricardo Andrade¹  Krug, Jéssica Lenita¹  Alberton, Michele Debiasi¹  Ribeiro, Suzi Meneses¹  Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil¹  Tenfen, Adrielli¹  Universidade Regional de Blumenau, Blumenau, Brazil¹  Paganelli, Camila Jeriane¹  Bianco, Éverson Miguel¹  Kroger, Aline¹ 

关键词:  Elatol;    Dolastane diterpene;    Antimollicutes activity;    Antioxidant activity;    Anticholinesterase activity;   

DOI  :  10.1016/j.bjp.2015.07.018

来源: Sociedade Brasileira de Farmacognosia

【 摘 要 】

This work describes the antimicrobial, antioxidant and anticholinesterase activities in vitro of organic extracts from fourteen seaweeds, eleven sponges, two ascidians, one bryozoan, and one sea anemone species collected along the Brazilian and Spanish coast, as well as the isolation of the diterpene (4R, 9S, 14S)-4α-acetoxy-9β,14α-dihydroxydolast-1(15),7-diene (1) and halogenated sesquiterpene elatol (2). The most promising antimicrobial results for cell wall bacteria were obtained by extracts from seaweeds Laurencia dendroidea and Sargassum vulgare var. nanun (MIC 250 μg/ml), and by the bryozoan Bugula neritina (MIC 62.5 μg/ml), both against Staphylococcus aureus. As for antimollicutes, extracts from seaweeds showed results better than the extracts from invertebrates. Almost all seaweeds assayed (92%) exhibited some antimicrobial activity against mollicutes strains (Mycoplasma hominis,Mycoplasma genitalium,Mycoplasma capricolum and Mycoplasma pneumoniae strain FH). From these seaweeds, A1 (Canistrocarpus cervicornis), A11 (Gracilaria sp.) and A4 (Lobophora variegata) showed the best results for M. pneumoniae strain FH (MIC 250 μg/ml). Furthermore, compounds 1 and 2 were also assayed against mollicutes strains M. hominis,M. genitalium,M. capricolum,M. pneumoniae strain 129 and M. pneumoniae strain FH, which showed MIC > 100 μg/ml. Antioxidant activities of extracts from these marine organisms were inactive, except for E7 (from sponge Ircinia sp.), which exhibited moderated antioxidant activities for two methods assayed (IC50 83.0 ± 0.1 μg/ml, and 52.0 ± 0.8 mg AA/g, respectively). Finally, for the anticholinesterase activity, all the 29 samples evaluated (100%) exhibited some level of activity, with IC50 < 1000 μg/ml. From these, seaweeds extracts were considered more promising than marine invertebrate extracts [A10 (IC50 14.4 ± 0.1 μg/ml), A16 (IC50 16.4 ± 0.4 μg/ml) and A8 (IC50 14.9 ± 0.5 μg/ml)]. The findings of this work are useful for further research aiming at isolation and characterization of active compounds.

【 授权许可】

Unknown