Biofilm inhibition activity of compounds isolated from two Eunicea species collected at the Caribbean Sea

Revista Brasileira de Farmacognosia

Gamba, Luis Reina¹  Hernández, Leonardo Castellanos¹  Arévalo-Ferro, Catalina¹  Universidad Nacional de Colombia, Bogotá D.C., Colombia¹  Beltrán, Carmenza Duque¹  Díaz, Yenny Martínez¹  Rodríguez, Freddy Ramos¹  Laverde, Gina Vanegas¹  Wandurraga, Humberto Mayorga¹ 

关键词:  Anti-biofilm;    Eunicea fusca;    Eunicea sp.;    Lipid compounds;    Terpenoids;    Marine natural products;   

DOI  :  10.1016/j.bjp.2015.08.007

来源: Sociedade Brasileira de Farmacognosia

【 摘 要 】

Biofilm has a primary role in the pathogenesis of diseases and in the attachment of multicellular organisms to a fouled surface. Because of that, the control of bacterial biofilms has been identified as an important target. In the present study, five lipid compounds isolated from soft coral Eunicea sp. and three terpenoids together with a mixture of sterols from Eunicea fusca collected at the Colombian Caribbean Sea showed different effectiveness against biofilm formation by three marine bacteria associated with immersed fouled surfaces, Ochrobactrum pseudogringnonense,Alteromona macleodii and Vibrio harveyi, and against two known biofilm forming bacteria, Pseudomonas aeruginosa ATCC 27853 and Staphylococcus aureus ATCC 25923. The pure compounds were characterized by NMR, HRESI-MS, HRGC-MS and optical rotation. The most effective compounds were batyl alcohol (1) and fuscoside E peracetate (6), acting against four strains without affecting their microbial growth. Compound 1 showed biofilm inhibition greater than 30% against A. macleodii, and up to 60% against O. pseudogringnonense,V. harveyi and S. aureus. Compound 6 inhibited O. pseudogringnonense and V. harveyi between 25 and 50%, and P. aeruginosa or S. aureus up to 60% at 0.5 mg/ml. The results suggest that these compounds exhibit specific biofilm inhibition with lower antimicrobial effect against the bacterial species assayed.

【 授权许可】

Unknown