Anti-inflammatory sesquiterpene lactones from Tithonia diversifolia trigger different effects on human neutrophils

Revista Brasileira de Farmacognosia

Rocha, Bruno A.¹  Oliveira, Carine E. de¹  Universidade de São Paulo, Ribeirão Preto, Brazil¹  Gasparoto, Thais H.¹  Universidade de São Paulo, Bauru, Brazil¹  Da Costa, Fernando B.¹  Campanelli, Ana P.¹  Chagas-Paula, Daniela A.¹  Oliveira, Rejane B. de¹  Abe, Aneli E.¹  Dalboni, Thalita M.¹ 

关键词:  Tagitinin A;    Tagitinin C;    Tagitinin F;    Human neutrophils;    Anti-inflammatory activity;   

DOI  :  10.1016/j.bjp.2015.01.005

来源: Sociedade Brasileira de Farmacognosia

【 摘 要 】

The tagitinins isolated of Tithonia diversifolia (Hemsl.) A. Gray, Asteraceae, are the most studied sesquiterpene lactones due to their wide spectrum of pharmacologic activities, especially related with nuclear factor-kappa B inhibition. Nevertheless, detailed studies about the mechanism of action of its active compounds are still lacking. Neutrophils perform a fundamental role in the inflammatory response to several etiologic factors. However, the effect of tagitinins on human neutrophil is not yet clearly known. We investigated the role of tagitinin C (1), tagitinin F (2) and tagitinin A (3) in activation and survival of human neutrophils to establish possible effects in their mechanisms of inflammation. Human neutrophils were purified from the peripheral blood and cultivated with tagitinins C (1), F (2) and A (3) in the presence or not of Escherichia coli lipopolysaccharide. The enzymatic activity, apoptosis and secretion of cytokines rate were determined after 18 h. Lipopolysaccharide-induced myeloperoxidase activity of human neutrophils was significantly inhibited only by tagitinin F (2). Apoptosis of neutrophils was increased in the presence of tagitinin C (1), and it occurred independently of the presence of lipopolysaccharide or dexamethasone. Tagitinins C (1), F (2) and A (3) decrease lipopolysaccharide-induced interleukin-6, interleukin-8 and Tumor necrosis factor alpha production by human neutrophils. Together, these results indicate that tagitinins exhibit anti-inflammatory action on human neutrophils. However, tagitinin F (2) was the only sesquiterpene lactone that decreased secretion of inflammatory products by neutrophils without inducing neutrophil apoptosis.

【 授权许可】

Unknown