Growth and microcystin production of a Brazilian Microcystis aeruginosa strain (LTPNA 02) under different nutrient conditions

Revista Brasileira de Farmacognosia

Friedrich-Schiller University of Jena, Jena, Brazil¹  Colepicolo, Pio¹  Pinto, Ernani¹  Saarland University, Saarbrücken, Brazil¹  Bortoli, Stella¹  Universidade Federal de São Paulo, Diadema, Brazil¹  Oliveira-Silva, Diogo¹  Dörr, Felipe A.¹  Universidade de São Paulo, São Paulo, Brazil¹  Volmer, Dietrich A.¹  Hans Knöll Institute, Jena, Germany¹  Krüger, Thomas¹ 

关键词: Cyanobacteria;    Microcystis sp.;    Microcystin;    LC-MS/MS;   

DOI  :  10.1016/j.bjp.2014.07.019

来源: Sociedade Brasileira de Farmacognosia

【 摘 要 】

Cyanobacteria are prokaryotic and photosynthetic organisms, which can produce a wide range of bioactive compounds with different properties; including a variety of toxic compounds, also known as cyanotoxins. In this work, we describe the isolation of seven cyanobacterial strains from two reservoirs in São Paulo State, Brazil. Seven different chemical variants of microcystins (MC-RR, MC-LR, MC-YR, MC-LF, MC-LW, and two demethylated variants, dm-MC-RR and dm-MC-LR) were detected in three of the ten isolated strains. One particular Microcystis aeruginosa strain (LTPNA 02) was chosen to evaluate its growth by cell count, and its toxin production under seven different nutritional regimes. We observed different growth behaviors in the logarithmic growth period for only three experiments (p <0.05). The total growth analysis identified four experiments as different from the control (p < 0.01). Three microcystin variants (MC-RR, MC-LR and MC-YR) were quantified by liquid chromatography-tandem mass spectrometry. At the experimental end, the toxin content was unchanged when comparing cell growth in ASM-1 (N:P = 1), MLA and BG-11 (N:P = 10) medium. In all other experiments, the lowest microcystin production was observed from cells grown in Bold 3N medium during the exponential growth phase. The highest microcystin content was observed in cultures using BG-11(N:P = 100) medium.

【 授权许可】

Unknown