Extracts from the leaves of Campomanesia velutina inhibits production of LPS/INF-γ induced inflammatory mediators in J774A.1 cells and exerts anti-inflammatory and antinociceptive effects in vivo

Revista Brasileira de Farmacognosia

Rezende, Simone A.¹  Paula, Carmem A.¹  Sobral, Marcos E.G.¹  Guimarães, Dênia A. Saúde¹  Michel, Marcela C.P.¹  Universidade Federal de Ouro Preto, Ouro Preto, Brazil¹  Guimarães, Andrea Grabe¹  Universidade Federal de São João Del-Rei, Brazil¹ 

关键词: Campomanesia velutina;    Myricitrin;    Anti-inflammatory;    Antinociceptive;    Inflammatory mediators;   

DOI  :  10.1590/S0102-695X2013000600010

来源: Sociedade Brasileira de Farmacognosia

【 摘 要 】

Campomanesia velutina (Cambess) O. Berg, Myrtaceae, popularly known as "gabiroba" or "guavira", is used in traditional Brazilian medicine to treat several diseases, including inflammation and rheumatism. Extraction and isolation from leaves of the plant afforded the active compound myricetin 3-O-rhamnoside, also known as myricitrin. The ethanolic extract of leaves of C. velutina and its ethyl acetate and methanolic fractions were evaluated in inflammation (carrageenan-induced paw oedema) and analgesic models (acetic acid-induced abdominal writhing and hot plate test). Moreover, the ethanolic extract, its fractions and the isolated compound were also in vitroevaluated for their ability to modulate NO, TNF-α and IL-10 production from J774A.1 macrophages stimulated by LPS/IFN-γ. In vivo assays showed remarkable anti-inflammatory activity of ethanolic extract, ethyl acetate and methanolic fractions. The antinociceptive activity of ethanolic extract and A was demonstrated in acetic acid-induced abdominal writhing test. In vitro assays demonstrated that ethyl acetate and methanolic fractions fraction and myricitrin inhibited NO production from macrophages J774A.1. Also Myricitrin induced production of IL-10 anti-inflammatory cytokine. None of the samples was able to inhibited TNF-α production. The results demonstrated for the first time the anti-inflammatory and antinociceptive activity of C. velutina.

【 授权许可】

Unknown