Anti-candidal activity of Astragalus verus in the in vitro and in vivo guinea pig models of cutaneous and systemic candidiasis

Revista Brasileira de Farmacognosia

Gholamine, Babak¹  Khanlari, Ali¹  Kermanshah University of Medical Sciences, Iran¹  Karimi, Isaac¹  Mikaeili, Ali¹  Shamspur, Tayebeh¹  Modaresi, Masoud¹  Shahid Bahonar University of Kerman, Iran¹  Razi University, Kermanshah, Iran¹ 

关键词: Astragalus verus;    candidiasis;    chemical analysis;    guinea pig;    Kurdish ethnomedicine;   

DOI  :  10.1590/S0102-695X2012005000035

来源: Sociedade Brasileira de Farmacognosia

【 摘 要 】

This study was design to evaluate the anti-candidal activity of Astragalus verus Olivier, Fabaceae (Av). The GC/MS analysis of essential oils of Av showed that aqueous extract contains thymol while hexadecanoic acid, 1,2-benzenedicarboxylic acid, diisooctyl ester and phytol were found as major components of methanol and acetone extracts. The aqueous extract showed anti-candidal activity in the concentration 320 mg/mL using disc diffusion method and its minimum inhibitory concentration (MIC) was 160 mg/mL. To induce cutaneous candidiasis, the dorsum of immunocompromised guinea pigs was infected with Candida albicans and animals were divided into five groups (n=5 for each): NC, received a vehicle; PC, received topical ketoconazole 2% and three other groups which received topical 10, 20 and 40% aqueous extract of Av. On ninth day postinfection, skins were cultured and colony forming unite per gram (CFU/g) skin was recorded. Systemic candidiasis was obtained by intravenous inoculation of C. albicans, 4000 CFU/g body weight. Here, animals have been divided into five groups like cutaneous candidiasis but their medications have been delivered in drinking water for ten days before induction of infection. On second day postinfection, all internal tissues were taken for determining CFU/g tissue. The aqueous extract (40%) prevented heavy burden of C. albicans in tissues and skin in oral and topical application, respectively. The results indicate that Av represents a potential source of anti-candidal drug.

【 授权许可】

Unknown