Tephrosia sinapou extract reduces inflammatory leukocyte recruitment in mice: effect on oxidative stress, nitric oxide and cytokine production

Revista Brasileira de Farmacognosia

Martinez, Renata M.¹  Baracat, Marcela M.¹  Zarpelon, Ana C.¹  Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Brazil¹  Universidade Estadual de Londrina, Brazil¹  Casagrande, Rubia¹  Georgetti, Sandra R.¹  Moreira, Isabel C.¹  Andrei, Cesar C.¹  Vicentini, Fabiana T. M. C.¹  Verri Jr, Waldiceu A.¹  Fonseca, Maria J. V.¹  Universidade Tecnológica Federal do Paraná, Brazil¹  Zimermann, Vanessa V. M.¹ 

关键词: cytokine;    inflammation;    neutrophil;    nitric oxide;    oxidative stress;    Tephrosia sinapou;   

DOI  :  10.1590/S0102-695X2012005000006

来源: Sociedade Brasileira de Farmacognosia

【 摘 要 】

Tephrosia toxicaria (Sw.) Pers., which is currently known as T. sinapou (Buc'hoz) A. Chev., Fabaceae, is a source of compounds such as flavonoids, however, few studies addressed the anti-inflammatory and antioxidant effects of T. sinapou. Therefore, we evaluated the antioxidant mechanisms of the T. sinapou ethyl acetate extract in vitro, and whether the extract affects leukocyte recruitment in four models of inflammation and the involvement of nitric oxide and cytokines in its mechanism. In vitro, it was observed that the extract presented hydrogen donating ability to 2,2-diphenyl-1-picryl-hydrazyl radical (DPPH), 2,2'-azino-di-(3-ethylbenzthiazoline-6-sulphonic acid) radical (ABTS+), and also efficiently inhibited iron-dependent and independent lipid peroxidation and iron chelation assays. In vivo, it inhibited the recruitment of total leukocytes and neutrophil induced by carrageenin, zymosan, glycogen and lipopolysaccharide in the peritoneal cavity of mice. Two mechanisms were detected: 1) T. sinapou effect on leukocyte recruitment depends on nitric oxide since was dose-dependently inhibited by treatment with L-NAME (nitric oxide synthase inhibitor), and 2) the extract also inhibited the production of crucial cytokines for the leukocyte recruitment; tumor necrosis factor α and interleukin-1β. Concluding, T. sinapou ethyl acetate extract reduces oxidative stress in vitro, and inflammatory leukocyte recruitment by a mechanism related to inhibition of cytokine production, and in a nitric oxide dependent manner in vivo.

【 授权许可】

Unknown