Antiherpetic activity of a flavonoid fraction from Ocotea notata leaves

Revista Brasileira de Farmacognosia

Romanos, Maria Teresa V.¹  Universidade Federal do Rio de Janeiro, Brazil¹  Rocha, Leandro¹  Santos, Marcelo G.¹  Universidade Federal Fluminense, Brazil¹  Garrett, Rafael¹  Silva, Antonio Jorge R. da¹  Borges, Ricardo M.¹ 

关键词: antiherpes;    flavonoids;    Ocotea notata;    HSV-1;    HSV-2;   

DOI  :  10.1590/S0102-695X2012005000003

来源: Sociedade Brasileira de Farmacognosia

【 摘 要 】

This study describes the isolation of a flavonoid fraction from leaves of Ocotea notata (Nees & Mart.) Mez, Lauraceae, the identification of six major compounds (an A-type proanthocyanidin trimer [3], isoquercitrin [4], reynoutrin [5], miquelianin [6], quercitrin [7], afzelin [8]) and four minor compounds (catechin [1], epicatechin [2], quercetin [9], kaempferol [10]) present in the fraction and its activity against the Herpes simplex virus type 1 (HSV-1) and type 2 (HSV-2). The 50% effective concentrations values (EC50) calculated from the dose-response curve and the selectivity indices (SI) against the virus were: EC50 35.8 µg/mL and SI 5.5 to HSV-1 and EC50 23.5 µg/mL and SI 8.5 to HSV-2. The flavonoid fraction was more active against HSV-2 than HSV-1. The mechanisms of antiviral action of the flavonoid fraction against the virus were also evaluated. The percentage inhibition (PI) obtained for HSV-2 was higher than 90% in the following assays: virucidal, pre-treatment of cells, treatment of cells after viral adsorption and treatment of cells after viral penetration. For HSV-1, the flavonoid fraction had no effect in pre-treatment of cells and showed 60% of inhibition in virucidal assay.

【 授权许可】

Unknown