Wogonin and neobaicalein from Scutellaria litwinowii roots are apoptotic for HeLa cells

Revista Brasileira de Farmacognosia

Emami, Seyed Ahmad¹  Mousavi, Seyed Hadi¹  Tayarani-Najarani, Zahra¹  Asili, Javad¹  Mirzaee, Alireza¹  Parsaee, Heydar¹  Mashhadian, Naser Vadati¹  University of Medical Sciences, Mashhad, Iran¹ 

关键词: apoptosis Lamiaceae neobaicalein Scutellaria litwinowii wogonin;   

DOI  :  10.1590/S0102-695X2011005000161

来源: Sociedade Brasileira de Farmacognosia

【 摘 要 】

Chemical investigation on the CH2Cl2 fraction of the Scutellaria litwinowii Bornm. & Sint., Lamiaceace, root extract for the first time resulted in the isolation of wogonin, and neobaicalein. These compounds were evaluated for their cytotoxicity towards HeLa cell lines and lymphocytes. Meanwhile, the role of apoptosis was explored in this toxicity. The cells were cultured in RPMI medium and incubated with different concentrations of isolated flavonoids. Cell viability was quantified by MTS assay. Apoptotic cells were determined using propidium iodide staining of DNA fragmentation by flow cytometry (sub-G1peak). Wogonin, and neobaicalein inhibited the growth of malignant cells in a dose-dependent manner. The IC50 values of 46.62 and 79.34 µM were, respectively, found for neobaicalein and wogonin against HeLa cells after 48 h of treatment. Neobaicalein induced a sub-G1 peak in the flow cytometry histogram of treated cells compared to control cells indicating that apoptotic cell death is involved in neobaicalein toxicity. Neobaicalein exerts cytotoxic and pro-apoptotic effects in HeLa cell lines and could be considered as a potential chemotherapeutic agent in cancer treatment.

【 授权许可】

Unknown