In vivo and in vitro anti-inflammatory activity of red clover Trifolium pratense dry extract

Revista Brasileira de Farmacognosia

Ceolato, Paula C.¹  Dall'Agnol, Miguel¹  Universidade Federal do Rio Grande do Sul, Brazil¹  Schapoval, Elfrides E. S.¹  Ramos, Graziele P.¹  Zuanazzi, José A. S.¹  Apel, Miriam A.¹  Morais, Cláudia B. de¹ 

关键词: Trifolium pratense;    isoflavones;    anti-inflammatory;    chemotaxis;    leukocyte migration;    rat paw edema;   

DOI  :  10.1590/S0102-695X2011005000200

来源: Sociedade Brasileira de Farmacognosia

【 摘 要 】

Red clover Trifolium pratense L., Fabaceae, contains four isoflavones, mainly formononetin and biochanin A, and in smaller concentrations, daidzein and genistein. These compounds have gained a lot interest due to its human health benefits, such as estrogenic and progestogenic activities, antioxidant, anti-cancer and others. The objective of this study was to determine in vivo and in vitro anti-inflammatory activity of red clover dry extract. The in vitro anti-inflammatory activity was assayed by the technique using the Boyden chamber method, evaluating the leukocyte migration inhibition (chemotaxis). The in vivo anti-inflammatory activity was tested by a carrageenan-induced rat paw edema test. The results of anti-inflammatory in vitro test showed that there was a significant inhibition of leukocyte migration at the concentrations of 100, 50, 25, 10 and 5 µg/mL of red clover dry extract, these doses resulted in 94.73, 95.39, 94.73, 84.68 and 78.75% of inhibition for each dose, respectively. The anti-inflammatory in vivo test resulted in a significant activity in both tested doses (100 and 50 mg/kg of red clover dry extract) and at each tested time. The average percentage of edema inhibition was 63.37%. The findings of this study suggested that red clover extract might be suitable for the treatment of inflammatory diseases.

【 授权许可】

Unknown