Phythochemical screening and antimicrobial activity phythochemical of essential oil from Lippia gracillis

Revista Brasileira de Farmacognosia

Siqueira, Jullyana de Souza¹  Rodrigues, Fabiola F. G.¹  Ruela, Ronaldo Sousa¹  Botelho, Marco Antônio¹  Martins, Karine T.¹  Veras, Helenicy N. H.¹  Lyra, Aarão¹  Bitu, Vanessa¹  Costa, José G. M. da¹  Queiroz, Dinalva Brito¹  Quintans-Junior, Lucindo J.¹  Coluchi, Giselle Gasparino¹ 

关键词: antimicrobial activity;    essential oil;    GC⁄MS;    Lippia gracillis;    monoterpene;    sesquiterpenes;   

DOI  :  10.1590/S0102-695X2011005000173

来源: Sociedade Brasileira de Farmacognosia

【 摘 要 】

The chemical composition of the essential oil obtained from the fresh and dried leaves of Lippia gracillis Schauer, Verbenaceae, was analyzed by gas chromatography and gas chromatography/mass spectrometry (GC⁄MS). The yield of essential oil extracted from the dried leaves was significantly higher (p<0.05) when compared to the fresh leaves. Seventeen components were identified. The monoterpenes and sesquiterpene hydrocarbons with 96.26% (w/w) of the total oil obtained of fresh leaves and 86.99% (w/w) of the total oil obtained of dried leaves were the principal compound groups. Thymol was observed dominant (44.42%; 21.3%), followed by carvacrol (22.21%; 21.30%), p-cymene (6.23%; 8.58%), α-pinene (5.65%; 19.42%), β-caryophyllene (5.61%; 3.57%) and other minor constitutes, respectively. Microbiological results obtained by agar diffusion method, micro dilution method and minimum inhibitory concentration (MIC) showed that the essential oil has a relevant antimicrobial activity against E. coli (ATCC 10536), E. coli (Ec 27), Pseudomonas aeruginosa (ATCC 15442), S. aureus (ATCC 12692) and S. aureus (Sa 358), with their inhibition zones ranging from 9 to 13 mm and the MIC ranging from 64 to 512 μg/mL.

【 授权许可】

Unknown