Validated high-performance liquid chromatographic method for the standardisation of Ptychopetalum olacoides Benth., Olacaceae, commercial extracts

Revista Brasileira de Farmacognosia

Reimberg, Maria Célia H.¹  Bolzani, Vanderlan da Silva¹  Silva, Dulce Helena S.¹  Bomfim, Giovani C. C.¹  Batista, Andrea N. de L.¹  Colombo, Renata¹  Burgos, Rosilene C. R.¹  Cavalheiro, Alberto J.¹  Anidro do Brasil Extrações Ltda, Botucatu, Brazil¹  Universidade Estadual Paulista, Araraquara, Brazil¹ 

关键词: HPLC-UV/PAD;    NMR;    Ptychopetalum olacoides;    marapuama;    vanillic acid.;   

DOI  :  10.1590/S0102-695X2010005000027

来源: Sociedade Brasileira de Farmacognosia

【 摘 要 】

Ptychopetalum olacoides Benth., Olacaceae, popularly known as marapuama or muirapuama or miriantã, is a species native to the Amazonian region of Brazil. Extracts of the bark of the plant have been used traditionally for its stimulating and aphrodisiac properties and currently commercialised by the herbal industry as constituents in a wide range of phytomedicines. Fractionation by open column chromatography followed by preparative HPLC-UV/PAD of the stem bark and of three commercial extracts of P. olacoides allowed the isolation of three components that were common to all extracts analysed, and these were identified by NMR to be vanillic acid, protocatechuic acid and theobromine. Vanillic acid, which has been proposed as a phytochemical marker for P. olacoides, was employed as an external standard in the development and validation of a rapid qualitative and quantitative HPLC assay for the analyte. The recoveries values of the developed method were 99.02% and the LOD and LOQ values were 0.033 and 0.11 mg.L-1, respectively. The described method may be applied to the standardisation of herbs, extracts or phytomedicines commercialised as marapuama.

【 授权许可】

Unknown