The Evaluation of the genotoxic potency of the Pyrostegia venusta (Ker Gawl.) Miers, Bignoneaceae, crude extract on bone marrow of mice

Revista Brasileira de Farmacognosia

Silva, Luciana P.¹  Magalhães, Eduardo A.¹  Universidade do Estado de Minas Gerais, Patos de Minas, Brasil¹  Universidade Estadual Paulista, Assis, Brasil¹  Silva Júnior, Gabriel J.¹  Silva, Regildo M. G.¹  de Campos, Thiago Augusto¹ 

关键词: micronucleus test;    chromosomal aberrations;    genotoxicity;    Pyrostegia venusta;    mice.;   

DOI  :  10.1590/S0102-695X2010000100014

来源: Sociedade Brasileira de Farmacognosia

【 摘 要 】

Pyrostegia venusta (Ker Gawl.) Miers, Bignoneaceae, is used in the treatment of vitiligo and other diseases, but its genotoxic effects are unknown. In this way, this study aimed to evaluate the genotoxic effect of extracts of P. venusta in mice using the micronucleus (MN) and chromosome aberration tests (CA). The plant was collected, selected, dried, pounded and extracted with ethanol. Mice weighing 40 g were divided in experimental and control groups. The experimental groups received different concentrations (50, 100, and 200 mg/kg body weight), by oral gavage. The negative control group (NC) received water. The positive control group (PC) received Ciclophosphamide® by v.i. It was performed the sacrifice, removal of bone marrow, homogenization and slides preparation. Frequency of micronucleated polychromatic erythrocytes (MNPCE) was: NC = 0.35±1.87; PC = 2.87±9.02; 50 = 0.09±0.83; 100 = 0.16±0.10 e 200 = 0.10±0.71. The CA test showed the frequency: NC = 0.12±1.87; PC = 0.62±5.61; 50 = 0.12±1.58; 100 = 0.072±0.54 e 200 = 0.124±1.64. The frequency of MNPCE of experimental controls was significantly lower when compared with NC, but it was statistically lower than PC's frequency. P.venusta didn't show genotoxicity activity.

【 授权许可】

Unknown