Antioxidant capacity and biological activity of essential oil and methanol extract of Hyptis crenata Pohl ex Benth

Revista Brasileira de Farmacognosia

Silva, Joyce Kelly R. da¹  Andrade, Eloísa Helena A.¹  Maia, José Guilherme S.¹  Universidade Federal do Pará, Belém, Brazil¹  Rebelo, Monaliza M.¹ 

关键词: Hyptis crenata;    Lamiaceae;    salva-do-marajó;    essential oil;    antioxidant activity;    total phenolic content;    cytotoxicity.;   

DOI  :  10.1590/S0102-695X2009000200009

来源: Sociedade Brasileira de Farmacognosia

【 摘 要 】

The essential oils of fresh and dried leaves and fine stems of Hyptis crenata furnished the following yields: 1.4% and 0.9%. The main volatile constituents were α-pinene (22.0%; 19.5%), 1,8-cineole (17.6%; 23.2%), β-pinene (17.0%; 13.8%), camphor (4.7%; 11.6%), limonene (5.4%; 4.4%) and γ-terpinene (3.5%; 2.4%), totalizing more than 70% in the oils. The DPPH radical scavenging activity (EC50, 16.7 + 0.4 µg/mL) of the methanol extract was comparable to BHT (19.8 ± 0.5 µg/mL) showing a significant antioxidant activity. The oils showed low activities. The amount of total phenolics (TP, 373.0 + 15.9 mg GAE/g) and trolox equivalent (TEAC, 226.8 + 0.5 mg TE/g) confirmed the antioxidant activity of the methanol extract that can be attributed to the presence of polar phenolic compounds. In the brine shrimp bioassay the lethal concentrations (LC50) for the oil and methanol extract were 6.7 + 0.2 µg/mL and 13.0 + 3.7 µg/mL, respectively, providing important evidence of their biological activities.

【 授权许可】

Unknown