Cytotoxicity of Wedelia paludosa D.C. extracts and constituents

Revista Brasileira de Farmacognosia

Universidade Estadual do Sudoeste da Bahia, Itapetinga, Brazil¹  Universidade Federal de Minas Gerais, Belo Horizonte, Brazil¹  Brandão, Geraldo C.¹  Batista, Ronan¹  Braga, Fernão C.¹  Oliveira, Alaíde B.¹ 

关键词: Wedelia paludosa;    Asteraceae;    cytotoxic activity;    brine shrimp lethality bioassay;    kaurenoic acid;    grandiflorenic acid;    HPLC.;   

DOI  :  10.1590/S0102-695X2009000100009

来源: Sociedade Brasileira de Farmacognosia

【 摘 要 】

Wedelia paludosa D.C. (Asteraceae) is an ornamental species occurring in many regions of Brazil. Aiming to find new cytotoxic compounds, the hydromethanol extract of W. paludosa (HME), as well as the dichloromethane (DF) and water (WF) fractions resulting from its partition, were submitted to the brine shrimp lethality bioassay (BSLB) in order to evaluate their cytotoxicity. Dichloromethane fraction (DF) was shown to be the most cytotoxic fraction (LC50 = 140.6 μg/mL), and its analysis by reversed phase high performance liquid chromatography (RP-HPLC) revealed ent-kaurenoic (1, 6.22 ± 0.23%) and grandiflorenic (2, 3.22 ± 0.31%) acids as important constituents. HME (LC50 = 980 μg/mL), DF (LC50 = 140.6 μg/mL), 1 (LC50 = 15.9 μg/mL) and 2 (LC50 = 29.8 μg/mL) were found to be cytotoxic, while the water fraction (WF, LC50 >> 1000 μg/mL) was inactive. As conclusion, the cytotoxicity observed for HME and DF is mainly due to the presence of 1 and 2 in their constitution.

【 授权许可】

Unknown