Revista Brasileira de Farmacognosia | |
Cytotoxicity of Wedelia paludosa D.C. extracts and constituents | |
Universidade Estadual do Sudoeste da Bahia, Itapetinga, Brazil1  Universidade Federal de Minas Gerais, Belo Horizonte, Brazil1  Brandão, Geraldo C.1  Batista, Ronan1  Braga, Fernão C.1  Oliveira, Alaíde B.1  | |
关键词: Wedelia paludosa; Asteraceae; cytotoxic activity; brine shrimp lethality bioassay; kaurenoic acid; grandiflorenic acid; HPLC.; | |
DOI : 10.1590/S0102-695X2009000100009 | |
来源: Sociedade Brasileira de Farmacognosia | |
【 摘 要 】
Wedelia paludosa D.C. (Asteraceae) is an ornamental species occurring in many regions of Brazil. Aiming to find new cytotoxic compounds, the hydromethanol extract of W. paludosa (HME), as well as the dichloromethane (DF) and water (WF) fractions resulting from its partition, were submitted to the brine shrimp lethality bioassay (BSLB) in order to evaluate their cytotoxicity. Dichloromethane fraction (DF) was shown to be the most cytotoxic fraction (LC50 = 140.6 μg/mL), and its analysis by reversed phase high performance liquid chromatography (RP-HPLC) revealed ent-kaurenoic (1, 6.22 ± 0.23%) and grandiflorenic (2, 3.22 ± 0.31%) acids as important constituents. HME (LC50 = 980 μg/mL), DF (LC50 = 140.6 μg/mL), 1 (LC50 = 15.9 μg/mL) and 2 (LC50 = 29.8 μg/mL) were found to be cytotoxic, while the water fraction (WF, LC50 >> 1000 μg/mL) was inactive. As conclusion, the cytotoxicity observed for HME and DF is mainly due to the presence of 1 and 2 in their constitution.
【 授权许可】
Unknown
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