Chemical composition of the fixed oil of Croton cajucara and its antifungical properties

Revista Brasileira de Farmacognosia

Universidade Federal do Amazonas, Manaus, Brasil¹  Cortez, Janaína K.P.C.¹  Universidade Federal do Rio Grande do Norte, Natal, Brasil¹  Leal, Rosélia de Sousa¹  Universidade Federal Rural do Rio de Janeiro, Seropédica, Brasil¹  Souza, Marco André A.¹  Maciel, Maria Aparecida M.¹  Veiga Jr, Valdir F.¹  Souza, Sonia R.¹  Dantas, Tereza N. Castro¹ 

关键词: Croton cajucara;    stem bark;    fixed oil;    chemical constituents;    antifungical properties.;   

DOI  :  10.1590/S0102-695X2006000500004

来源: Sociedade Brasileira de Farmacognosia

【 摘 要 】

This paper focused on the separation of the fixed oil (FO) from the stem bark of Croton cajucara obtained from the conventional approach extraction with organic solvents [followed by chromatography column (CC)] and supercritical fluid extraction (SFE, carried out with CO2) and also, on the antifungical properties of the FO obtained by CC procedure (FO-CC). The FO contents were 3.14% for FO-CC versus 1.20% in the FO-SFE process. The esterified fraction F1 and non-esterified fractions F2, F3 and F4 obtained from FO-CC after a new CC procedure were analyzed by HRGC-MS. The identification of the chemical composition of FO-CC was made by comparison with MS literature data, computer matching with the Wiley library and by comparison of their kovats indices with the literature. Fraction F1 showed 70% of sesquiterpene components, among them a-copaene (20.1%) and ciperene (21.8%) as major compounds. Fraction F2 was rich in minor oxygenated sesquiterpenes, among them linalool. Meanwhile fraction F3 showed fat acids, steroids and the bioactive clerodane type-diterpene trans-crotonin, cis-cajucarin B (c-CJC-B) and trans-cajucarin B (t-CJC-B). Fractions F3 and F4 showed as major constituents c-CJC-B and t-CJC-B with 40% of the total contents. The biological effect of the FO-CC was evaluated in the in vitro development of the phytopatogen fungi such as Fusarium oxysporum, Rhizoctonia solani and Sclerotium rolfsii. Significant inhibitory effect of the tested fungi (at 0.2 mg.mL-1 dosage) were proved.

【 授权许可】

Unknown