Photocolorimetric Biosensor for Detection of Cholinergic Organophosphorus Compounds

Defence Science Journal

Emil Halámek¹  Kamila Vymazalová³  Jiří Kadlčák² 

[1] Detection and Analytical Department of Military Technical Institute of Protection;University of Defense, the Institute of NBC Defence;Military Technical Institute of Protection

关键词: Nerve agent;    Ellman´s reaction;    tape biosensor;    colorimetric detection;   

DOI  :  

学科分类：社会科学、人文和艺术（综合）

来源: Defence Scientific Information & Documentation Centre

【 摘 要 】

To detect nerve agents in practice, the analytical methods such as gas, liquid and thin-layer chromatography, mass spectrometry or capillary electrophoresis are usually used. Apart from these analytical methods, we developed an analytical device (tape photocolorimetric biosensor) based on the modified Ellman's cholinesterase biochemical reaction for multidetection of cholinergic organophosphorus compounds. Enzyme butyrylcholinesterase was used as a biorecognizing component and its activity was evaluated by red, blue, green (RGB) sensor. This method eliminates errors in the evaluation and provides automatic data collection with their subsequent evaluation. The unique method of dosing allows appropriate dispensing of reagents in microlitres volumes and the whole system is simple to operate. Suitability of the constructed biosensors was evaluated using the six organophosphates (Tabun, sarin, Soman, cyclosin, VX and R33 compound). Biosensor showed the ability to measure substances at concentrations ranging between ~ 1×10-8 mg/l - 1×10-6 mg/l in the air, according to their inhibition effect. Defence Science Journal, 2012, 62(6), pp.399-403 , DOI:http://dx.doi.org/10.14429/dsj.62.2589

【 授权许可】

Unknown