Rapid Detection of Ricin by Sensitising Carboxylated Latex Particles by Ricin Antibodies

Defence Science Journal

G.P. Rai¹  Om Kumar¹  R. Vijayaraghavan¹  M.M. Parida¹ 

[1] Defence Research & Development Establishment, Gwalior

关键词: Ricin;    sensitised latex particles;    immunisation protocol;    immunogenic glycoprotein;    toxin. toxic lectin;    latex agglutination test;    protein inhibitor;    detection;    enzyme-linked immunosorbent assay;    ELISA;    optic-based biosensors;    ricin detection;    radioimmuno;   

DOI  :  

学科分类：社会科学、人文和艺术（综合）

来源: Defence Scientific Information & Documentation Centre

【 摘 要 】

Ricin is a highly toxic glycoprotein of Ricinus communis seeds. The toxin was purified and antisera was raised against ricin in rabbit. Polyclonal antibodies were covalently coupled through a water soluble carbodiimide to carboxylated latex particles in various concentrations (800 pg to 3200 pg proteid0.5 ml). Maximum antibodies binding was obtained at 2400 pg to 3200 pg proteid0.5 ml of 2 per cent (wtlvol) latex particles with a sensitivity of 200 pg toxin per test (9 pglml). The sensitivity of latex agglutination test increased as amount of protein bound to the latex particles increased. The optimum sensitivity of test was recorded when latex particles were sensitised with 2800 pg proteid0.5 ml of latex particles. The reagents were stable for one year without loss of its sensitivity. Developed latex agglutination test is rapid, sensitive, and also does not require trained personnel and costly equipment.

【 授权许可】

Unknown