Isolation of lactic acid bacteria exhibiting high scavenging activity for environmental hydrogen peroxide from fermented foods and its two scavenging enzymes for hydrogen peroxide

The Journal of General and Applied Microbiology

Youichi Niimura⁵  Masataka Uchino²  Akira Abe⁶  Sanae Okada⁴  Akio Watanabe⁵  Kouji Takeda⁵  Chiaki Kaneko⁵  Yasuhiro Hamada⁵  Takashi Matsumoto³  Naoto Tanaka⁴  Junichi Satoh⁵  Junichi Nakagawa¹  Shinya Kimata⁵ 

[1] Department of Food Science and Technology, Tokyo University of Agriculture;Department of Applied Biology and Chemistry, Tokyo University of Agriculture;Nodai Genome Research Center, Tokyo University of Agriculture;Nodai Culture Collection Center, Tokyo University of Agriculture;Department of Bio-Science, Tokyo University of Agriculture;Department of Ophthalmology, Sapporo Medical University

关键词: bacterial living cells;    hydrogen peroxide;    lactic acid bacteria;    NADH peroxidase;    scavenging of hydrogen peroxide;   

DOI  :  10.2323/jgam.62.75

学科分类：微生物学和免疫学

来源: Applied Microbiology, Molecular and Cellulrar Biosciences Research Foundation

【 摘 要 】

To obtain lactic acid bacteria that scavenge environmental hydrogen peroxide, we developed a specialized enrichment medium and successfully isolated Pediococcus pentosaceus Be1 strain from a fermented food. This strain showed vigorous environmental hydrogen peroxide scavenging activity over a wide range of hydrogen peroxide concentrations. High Mn-catalase and NADH peroxidase activities were found in the cell-free extract of the P. pentosaceus Be1 strain, and these two hydrogen peroxide scavenging enzymes were purified from the cell-free extract of the strain. Mn-catalase has been purified from several microorganisms by several researchers, and the NADH peroxidase was first purified from the original strain in this report. After cloning the genes of the Mn-catalase and the NADH peroxidase, the deduced amino acid sequences were compared with those of known related enzymes.

【 授权许可】

Unknown