Optimization of cellulase production by Penicillium oxalicum using banana agrowaste as a substrate

The Journal of General and Applied Microbiology

Kiran S. Kalia³  Shilpa P. Shah¹  Jagdish S. Patel² 

[1] Shree P. M. Patel Institute of Post Graduate Studies & Research in Science;Department of Biochemistry, PDPIAS, Charotar University of Science and Technology;BRD School of Biosciences, Sardar Patel University

关键词: alkali pretreatment;    banana agrowaste;    cellulase enzyme production;    Penicillium oxalicum;   

DOI  :  10.2323/jgam.61.35

学科分类：微生物学和免疫学

来源: Applied Microbiology, Molecular and Cellulrar Biosciences Research Foundation

【 摘 要 】

The purpose of this study was to produce a higher amount of cellulase by using an alternative carbon source, such as banana agrowaste, and to optimize the fermentation parameters for a high yield. In the present study, cellulase-producing Penicillium was isolated from a decaying wood sample. Different nutritional and environmental factors were investigated to assess their effect on cellulase production. The highest crude enzyme production was observed at a pH 6.0 and a temperature of 28°C in a medium that was supplemented with banana agrowaste as the carbon source. Pretreatment with 2N NaOH, at 7% substrate (banana agrowaste) concentration yielded the highest cellulase activity. Further to this, the effect of other parameters such as inoculum age, inoculum size, static and agitated conditions were also studied. It is concluded that Penicillium oxalicum is a powerful cellulase-producer strain under our tested experimental conditions using banana agrowaste as the carbon source.

【 授权许可】

Unknown