Concentrated biosynthesis of tobramycin by genetically engineered Streptomyces tenebrarius

The Journal of General and Applied Microbiology

Wenrong Hong¹  Shuping Wen¹  Hui Li¹  Jianping Xiao¹ 

[1] College of Biological Science and Technology, Fuzhou University

关键词: carbamoyltobramycin;    genetic engineering;    Streptomyces tenebrarius;    tobramycin;   

DOI  :  10.2323/jgam.60.256

学科分类：微生物学和免疫学

来源: Applied Microbiology, Molecular and Cellulrar Biosciences Research Foundation

【 摘 要 】

Tobramycin is an important broad spectrum aminoglycoside antibiotic widely used against severe Gram-negative bacterial infections. It is produced by base-catalyzed hydrolysis of carbamoyltobramycin (CTB) generated by S. tenebrarius. We herein report the construction of a genetically engineered S. tenebrarius for direct fermentative production of tobramycin by disruption of aprK and tobZ. A unique putative NDP-octodiose synthase gene aprK was disrupted to optimize the production of CTB, resulting in the blocking of apramycin biosynthesis and the obvious increase in CTB production of aprK disruption mutant S. tenebrarius ST316. Additional mutation on the carbamoyltransferase gene tobZ in S. tenebrarius ST316 generated a strain ST318 that produces tobramycin as a single metabolite. ST318 could be used for industrial fermentative production of tobramycin.

【 授权许可】

Unknown