In vitro assessment of Ag2O nanoparticles toxicity against Gram-positive and Gram-negative bacteria

The Journal of General and Applied Microbiology

Palaniyandi Rathinavelu Saravanan²  Tithi Agarwal¹  Harshita Negi²  Reeta Goel²  Mohd Ghulam Haider Zaidi¹ 

[1] Department of Chemistry, C.B.S.H., G. B. Pant University of Agriculture and Technology;Department of Microbiology, C.B.S.H., G. B. Pant University of Agriculture and Technology

关键词: Ag2O nanoparticles;    antibacterial activity;    ATP assay;    Bacillus circulans;    Escherichia coli;    microdilution method;   

DOI  :  10.2323/jgam.59.83

学科分类：微生物学和免疫学

来源: Applied Microbiology, Molecular and Cellulrar Biosciences Research Foundation

【 摘 要 】

In view of antibiotic resistance among pathogens, the present study is to address the toxicity of Ag2O nanoparticles against the Gram-positive and Gram-negative bacteria through in vitro assays. The preliminary screening by agar diffusion assay confirms the antibacterial activity of Ag2O nanoparticles against all the test bacteria. Comparative antibacterial activity of Ag2O nanoparticles and respective antibiotics reveals their broad range of activity and lower inhibitory dose against the used bacterial strains. Further, they can inhibit E. coli with an effective dose of 0.036 mg/ml within 1 h of exposure time as determined by luciferin based ATP assay. Moreover, the Ag2O nanoparticles exhibit higher antibacterial efficacy against Gram-negative bacteria than Gram-positive bacteria, as revealed by their MIC & MBC values. Therefore, Ag2O nanoparticles pave the way for a new generation of antibacterial agents against the emerging multidrug resistant pathogens.

【 授权许可】

Unknown