Isolation and identification of cultivable lactic acid bacteria in traditional yak milk products of Gansu Province in China

The Journal of General and Applied Microbiology

Xia Chen¹  HePing Zhang¹  QiuHua Bao¹  WenJun Liu¹  JianMin Qiao¹  Jie Yu¹  Fang Wang¹  ManJun Qing¹  TianSong Sun¹  WenYi Zhang¹  JiaChao Zhang¹  WeiHong Wang¹ 

[1] Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Inner Mongolia Agricultural University

关键词: identification;    isolation;    lactic acid bacteria;    PCR-DGGE;    16S rRNA gene sequence analysis;    yak milk products;   

DOI  :  10.2323/jgam.58.95

学科分类：微生物学和免疫学

来源: Applied Microbiology, Molecular and Cellulrar Biosciences Research Foundation

【 摘 要 】

Various traditional fermented yak milk and raw milk foods could be considered as an abundant resource for obtaining novel lactic acid bacteria (LAB) with unique properties. Eighty-eight samples of yak milk products were collected from Gansu Province in China. Three hundred and nineteen strains of LAB isolated from these samples were identified by phenotypic methods, 16S rRNA gene sequence analysis and PCR-Denaturing Gradient Gel Electrophoresis (PCR-DGGE) technology. Among the isolates, one hundred and sixty-four isolates (51.41% of the total) were classified under Lactobacilli, and one hundred and fifty-five (48.59%) belonged to cocci. All the isolates were classified to six genera (Lactobacillus, Lactococcus, Leuconostoc, Streptococcus, Enterococcus and Weissella) and twenty-one species. Lactobacillus helveticus (87 strains), Leuconostoc mesenteroides subsp. mesenteroides (49 strains), Streptococcus thermophilus (39 strains), Lactobacillus casei (31 strains) and Lactococcus lactis subsp. lactis (19 strains) were considered as the predominant populations in the yak milk products. The results showed that there were abundant genus and species LAB existing in yak milk products in Gansu Province in China. The obtained LAB pure cultures may be a valuable source for further starter selection.

【 授权许可】

Unknown