The Journal of General and Applied Microbiology | |
Engineered ACC deaminase-expressing free-living cells of Mesorhizobium loti show increased nodulation efficiency and competitiveness on Lotus spp. | |
Valeria P. Conforte1  Mariela Echeverria1  Ana B. Menéndez1  Viviana C. Lepek1  Cintia Sánchez1  Rodolfo A. Ugalde1  | |
[1] Instituto de Investigaciones Biotecnológicas, INTECH, Universidad Nacional de General San Martín | |
关键词: ACC deaminase; competitiveness; ethylene; lotus; Mesorhizobium loti; | |
DOI : 10.2323/jgam.56.331 | |
学科分类:微生物学和免疫学 | |
来源: Applied Microbiology, Molecular and Cellulrar Biosciences Research Foundation | |
【 摘 要 】
Ethylene inhibits the establishment of symbiosis between rhizobia and legumes. Several rhizobia species express the enzyme ACC deaminase, which degrades the ethylene precursor 1-cyclopropane-1-carboxilate (ACC), leading to reductions in the amount of ethylene evolved by the plant. M. loti has a gene encoding ACC deaminase, but this gene is under the activity of the NifA-RpoN-dependent promoter; thus, it is only expressed inside the nodule. The M. loti structural gene ACC deaminase (acdS) was integrated into the M. loti chromosome under a constitutive promoter activity. The resulting strain induced the formation of a higher number of nodules and was more competitive than the wild-type strain on Lotus japonicus and L. tenuis. These results suggest that the introduction of the ACC deaminase activity within M. loti in a constitutive way could be a novel strategy to increase nodulation competitiveness of the bacteria, which could be useful for the forage inoculants industry.
【 授权许可】
Unknown
【 预 览 】
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RO201912010139100ZK.pdf | 516KB | download |