Reclassification of a parathione-degrading Flavobacterium sp. ATCC 27551 as Sphingobium fuliginis

The Journal of General and Applied Microbiology

Kazuyoshi Kawahara¹  Atsushi Tanaka¹  Akira Yokota²  Jaewoo Yoon² 

[1] Department of Applied Material and Life Science, College of Engineering, Kanto Gakuin University;Institute of Molecular and Cellular Biosciences, The University of Tokyo

关键词: Flavobacterium;    glycosphingolipid;    organophosphorus hydrolase;    parathion;    Sphingobium fuliginis;   

DOI  :  10.2323/jgam.56.249

学科分类：微生物学和免疫学

来源: Applied Microbiology, Molecular and Cellulrar Biosciences Research Foundation

【 摘 要 】

A parathione-degrading bacterium isolated from rice field in the Philippines, Flavobacterium sp. ATCC 27551 (Sethunathan and Yoshida, 1973, Can. J. Microbiol., 19, 873-875), was re-examined chemotaxonomically and phylogenetically. The strain contained 2-hydroxymyristic acid (2-OH 14 : 0), cis-vaccenic acid (18 : 1 ω7c), and palmitic acid (16 : 0) as major cellular fatty acids, two kinds of glycosphingolipids, and ubiquinone-10 as a sole quinone component. The G+C content of genomic DNA of the strain was 65.9 mol%. The phylogenetic analyses of the 16S rRNA gene indicated that the strain was included in the family Sphingomonadaceae, and most closely related to Sphingobium fuliginis (98.0% similarity) and Sphingobium herbicidovorans (97.3%). The strain showed similar physiological characteristics and a moderate value of DNA-DNA relatedness to S. fuliginis. These data suggested it reasonable to conclude that strain ATCC 27551 was identified as S. fuliginis.

【 授权许可】

Unknown