Hfq regulates anti-oxidative ability in Vibrio parahaemolyticus

The Journal of General and Applied Microbiology

Akira Takahashi²  Zehong Su²  Masatake Akutagawa³  Masayuki Nakano¹  Tetsuro Koga²  Yutaka Nakaya¹  Xin Lian²  Akiko Hamamoto²  Kazuaki Mawatari²  Nagakatsu Harada¹  Takaaki Shimohata²  Yumi Harada² 

[1] Department of Nutrition and Metabolism, Institute of Health Biosciences, The University of Tokushima Graduate School;Department of Preventive Environment and Nutrition, Institute of Health Biosciences, The University of Tokushima Graduate School;Department of Electrical and Electronic Engineering, Institute of Socio Techno Sciences, The University of Tokushima Graduate School

关键词: catalase (CAT);    hfq;    superoxide dismutase (SOD);    Vibrio parahaemolyticus;   

DOI  :  10.2323/jgam.56.181

学科分类：微生物学和免疫学

来源: Applied Microbiology, Molecular and Cellulrar Biosciences Research Foundation

【 摘 要 】

Hfq plays a fundamental role in bacterial cell physiology. It can stimulate or repress the expression of certain target genes, and there is a possibility that Hfq regulates the oxidative stress response. However, how Hfq functions that in Vibrio parahaemolyticus remains speculative. In this paper, we explain the functions Hfq plays in V. parahaemolyticus in the gene expression of superoxide dismutase gene and catalase gene, comparing the hfq deletion mutant strain to the parental strain. The results show that the hfq deletion mutant V. parahaemolyticus has a stronger ability to resist H2O2. Superoxide dismutase (SOD) and catalase (CAT) activities in the hfq deletion mutant were remarkably higher than in the parental strain. Genetic experiments indicated that the gene expression of sod and kat was up-regulated in the mutant strain. These results indicate that Hfq down-regulates CAT and SOD activity, and Hfq is associated with the oxidative stress response.

【 授权许可】

Unknown