Molecular and biochemical characterization of three bacterial symbionts of fruit fly, Bactrocera tau (Tephritidae: Diptera)

The Journal of General and Applied Microbiology

Pankaj Sood¹  Pawan K. Mehta¹  Sarbjit S. Kanwar²  Prem N. Sharma³  Chandra S. Prabhakar¹  Vikas Kapoor³ 

[1] Department of Entomology, CSK Himachal Pradesh Krishi Vishvavidyalaya;Department of Microbiology, CSK Himachal Pradesh Krishi Vishvavidyalaya;Molecular Plant Pathology Lab., CSK Himachal Pradesh Krishi Vishvavidyalaya

关键词: bacterial symbionts;    Bactrocera tau;    fruit fly;    Klebsiella;    Pantoea;    phylogeny;    16S rRNA;   

DOI  :  10.2323/jgam.55.479

学科分类：微生物学和免疫学

来源: Applied Microbiology, Molecular and Cellulrar Biosciences Research Foundation

【 摘 要 】

Characterization of three bacterial symbionts (BC1, BC2 and BC3) of fruit fly Bactrocera tau including morphological, biochemical and 16S rDNA (rrs gene) analysis was done to determine their taxonomic position. Morphological and biochemical characterization placed two bacteria (BC1, Klebsiella oxytoca and BC2, Pantoea agglomerans) into family Enterobacteriaceae and the third one (BC3, Staphylococcus sp.) into family Staphylococcaceae. 16S rDNA gene sequence comparison with the available NCBI database sequences further confirmed the characterizations of bacterial symbionts. Molecular phylogeny of Klebsiella oxytoca and Pantoea agglomerans closely related to the other free living enterobacterial members with 74 to 93% sequence homology (genetic distance 0.000 to 0.085); however, they showed only 74 to 87% similarity with other insect symbionts (genetic distance 0.090 to 0.121). Staphylococcus sp. showed 94% sequence homology with other members of family Staphylococcaceae with the genetic distance of 0.013. Population of these symbionts in adult fruit flies increased exponentially up to the 10th day of adult emergence and thereafter it became almost constant.

【 授权许可】

Unknown