The Journal of General and Applied Microbiology | |
Functional cloning and predictive structural modeling of a novel esterase from Bacillus subtilis strain, RRL 1789 | |
Nishawar Jan1  Khurshid I. Andrabi1  Mahboob Ul Hussain1  Ghulam N. Qazi2  Vijeshwar Verma2  Kaiser Peerzada1  | |
[1] Department of Biotechnology, University of Kashmir;Indian Institute of Integrative Medicine (IIIM) | |
关键词: bacillus; cloning; esterases; genomic library; | |
DOI : 10.2323/jgam.55.317 | |
学科分类:微生物学和免疫学 | |
来源: Applied Microbiology, Molecular and Cellulrar Biosciences Research Foundation | |
【 摘 要 】
We have recently reported the purification and characterization of a novel esterase from the Bacillus subtilis strain. In the present study we report the genomic DNA cloning and predictive structural modeling of this novel esterase. Tributyrin- and Rhodamine B-based functional screen of a Bacillus subtilis genomic library led to the identification of a potential lipolytic gene. DNA sequence analysis of the cloned gene showed that it encodes a protein of 489 amino acid residues. Sequence homology search and multiple sequence alignment showed that the protein was highly homologous to known esterases. Secondary structure-driven multiple sequence alignment with the homologous esterase of known three-dimensional structures was performed and a 3D structure model of this enzyme was constructed. Based on the topological organization of the secondary structures, this protein belongs to the α/β hydrolase superfamily. Moreover, the presence of serine in the context of amino acid sequence G/A-X-S-X-G (with X an arbitary amino acid residue) in the protein indicates that it belong to the class of serine hydrolases of this superfamily.
【 授权许可】
Unknown
【 预 览 】
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