Functional expression of LZ-8, a fungal immunomodulatory protein from Ganoderma lucidium in Pichia pastoris

The Journal of General and Applied Microbiology

Changhua Shang¹  Chao Jiang¹  Mingwen Zhao¹  Yixin Ding¹  Qin Xue¹ 

[1] College of Life Sciences, Nanjing Agricultural University; Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture

关键词: express;    immunomodulatory activity;    LZ-8 protein;    Pichia pastoris;   

DOI  :  10.2323/jgam.54.393

学科分类：微生物学和免疫学

来源: Applied Microbiology, Molecular and Cellulrar Biosciences Research Foundation

【 摘 要 】

LZ-8, a fungal immunomodulatory protein found in Ganoderma lucidium, has been proposed to have therapeutic effects on cancer and autoimmune diseases. To develop an efficient and facile expression system supplying of rLZ-8 of high purity and stable activity for further study and applications, a full-length cDNA of lz8 gene was cloned into the pPIC9K to construct a yeast expression vector pPIC9K-lz8, then transformed to Pichia pastoris strain GS115. The molecular weight of the rLZ-8 was about 17 kDa. The yield of rLZ-8 reached up to 270 mg L-1 at optimal culture conditions. In vitro, the rLZ-8 was capable of hemagglutinating mouse red blood cells, but no such activity was observed toward human red blood cells, which were similar to the native LZ-8. The mouse splenocyte proliferation activity of rLZ-8 expressed in P. pastoris was much higher than the rLZ-8 expressed in E. coli. All these results suggested that the active rLZ-8 protein can be expressed efficiently in the P. pastoris expression system. This report may supply a new economic and convenient way for the application of LZ-8 protein.

【 授权许可】

Unknown