Lactobacillus rhamnosus L60, a potential probiotic isolated from the human vagina

The Journal of General and Applied Microbiology

Liliana M. Pascual¹  Cristina Pájaro¹  María B. Daniele¹  Francisco Ruiz¹  Walter Giordano²  Lucila Barberis¹ 

[1] Departamento de Microbiología e Inmunología;Departamento de Biología Molecular,Universidad Nacional de Río Cuarto

关键词: antimicrobial activity;    bacteriocin;    Lactobacillus rhamnosus;    probiotic;   

DOI  :  10.2323/jgam.54.141

学科分类：微生物学和免疫学

来源: Applied Microbiology, Molecular and Cellulrar Biosciences Research Foundation

【 摘 要 】

The vagina has been increasingly viewed as an "ecosystem" whose normal microflora help protect it from invading pathogens, including those that cause urinary tract infections and sexually transmitted diseases. We tested new strains of lactobacilli as potential probiotics for maintenance of urogenital tract health, as well as prevention and therapy of urogenital infections. A strain of lactobacilli isolated from the vagina of nonpregnant, healthy, premenopausal women was identified as Lactobacillus rhamnosus L60 by 16S rDNA sequence homology. L60 was evaluated for antimicrobial activity, in vitro antibiotic resistance, autoaggregation, surface hydrophobicity, co-aggregation with other bacterial species, hydrogen peroxide (H2O2) production, and bacterial adherence. It displayed a wide spectrum of antimicrobial activity against urogenital pathogens, and resistance to antibiotics commonly prescribed for infections caused by these pathogens. L60 produced H2O2, adhered to vaginal epithelial cells, co-aggregated with Escherichia coli and Candida albicans, and displayed self-aggregation. In view of these characteristics, L60 is considered a potential probiotic, and will be further evaluated for preventive and therapeutic application locally in the vaginal tract.

【 授权许可】

Unknown