Phylogenetic analysis of γ-proteobacteria inferred from nucleotide sequence comparisons of the house-keeping genes adk, aroE and gdh: Comparisons with phylogeny inferred from 16S rRNA gene sequences

The Journal of General and Applied Microbiology

Jean-Charles Côté¹  Hoon-Yong Lee¹ 

[1] Agriculture and Agri-Food Canada, Research Centre

关键词: adenylate kinase;    glucose-6-phosphate dehydrogenase;    phylogeny;    γ-proteobacteria;    shikimate dehydrogenase;    16S rRNA;   

DOI  :  10.2323/jgam.52.147

学科分类：微生物学和免疫学

来源: Applied Microbiology, Molecular and Cellulrar Biosciences Research Foundation

【 摘 要 】

Nucleotide sequence comparisons of three house-keeping genes, adenylate kinase (adk), shikimate dehydrogenase (aroE), and glucose-6-phosphate dehydrogenase (gdh), were used to infer the phylogeny of 33 γ-proteobacteria. Phylogenetic trees inferred from each gene, and from the concatenated sequences of all three genes, are, in general, similar to a 16S rRNA gene-inferred tree. Similar grouping of bacteria are revealed at the family, genus, species and strain levels in all five trees. The house-keeping genes, however, show a higher rate of nucleotide sequence substitutions. Consequently, they can possibly probe deeper branches of a phylogenetic tree than the 16S rRNA gene. However, because their nucleotide sequences are not as highly conserved among γ-proteobacteria, family- or genus-specific primers would need to be designed for the amplification of any of these three house-keeping genes. Since these genes are used in multilocus sequence typing, it is expected that the number of sequences publicly available for many taxa will increase over time proving them very useful either at complementing 16S rRNA-inferred phylogenies or for specific, targeted, phylogenetic analysis.

【 授权许可】

Unknown