Phylogenetic relationship within the Erythrobasidium clade: Molecular phylogenies, secondary structure, and intron positions inferred from partial sequences of ribosomal RNA and elongation factor-1α genes

The Journal of General and Applied Microbiology

Makiko Hamamoto³  Takahiko Nagahama¹  Koki Horikoshi¹  Shigeru Shimamura¹  Takashi Nakase² 

[1] Extremobiosphere Research Center, Japan Agency for Marine-Earth Science and Technology (JAMSTEC);Japan Collection of Microorganisms, RIKEN;Department of Life Sciences, School of Agriculture, Meiji University

关键词: elongation factor-1α;    Erythrobasidium clade;    intron;    rDNA;    rRNA secondary structure;   

DOI  :  10.2323/jgam.52.37

学科分类：微生物学和免疫学

来源: Applied Microbiology, Molecular and Cellulrar Biosciences Research Foundation

【 摘 要 】

Phylogenetic relationships within the Erythrobasidium clade as a lineage of the urediniomycetous yeasts were examined using partial regions of 18S rDNA, 5.8S rDNA, 26S rDNA, internal transcribed spacers (ITSs), and elongation factor (EF)-1α. Combined data analysis of all segments successfully yielded a reliable phylogeny and confirmed the cohesion of species characterized by Q-10(H2) as a major ubiquinone. Differences in secondary structure predicted for a variable region in 26S rDNA corresponded to major divergences in the phylogenetic tree based on the primary sequence. The common presence of a shortened helix in this region was considered to be evidence of monophyly for species with Q-10(H2), Sakaguchia dacryoides, Rhodotorula lactosa, and Rhodotorula lamellibrachiae, although it was not as strongly supported by the combined data tree. The information on intron positions in the EF-1α gene had potential usefulness in the phylogenetic inference between closely related species.

【 授权许可】

Unknown