A novel symbiotic nitrogen-fixing member of the Ochrobactrum clade isolated from root nodules of Acacia mangium

The Journal of General and Applied Microbiology

Achara Nuntagij²  Mikiko Abe⁴  Junzo Tsukahara⁴  Akihiro Suzuki⁴  Hiroyuki Sawada³  Yasuyoshi Nakagawa¹  Shiro Higashi⁴  Shiro Wakabayashi⁴  Toshiki Uchiumi⁴  Amy Ngom⁴  Somsak Kotepong² 

[1] Biological Resource Center, National Institute of Technology and Evaluation;Soil Microbiology Research Group, Division of Soil Science, Department of Agriculture;National Institute of Agro-Environmental Sciences;Laboratory of Plant and Microbe Interaction, Department of Chemistry and BioScience, Faculty of Science, Kagoshima University

关键词: Acacia mangium;    Bradyrhizobium;    determinate nodule;    nitrogen fixation;    nodule bacteria;    Ochrobactrum;    Rhizobium;    16S rRNA;   

DOI  :  10.2323/jgam.50.17

学科分类：微生物学和免疫学

来源: Applied Microbiology, Molecular and Cellulrar Biosciences Research Foundation

【 摘 要 】

Ten strains of root nodule bacteria were isolated from the nodules of Acacia mangium grown in the Philippines and Thailand. Partial sequences (approx. 300 bp) of the 16S rRNA gene of each isolate were analyzed. The nucleotide sequences of strain DASA 35030 indicated high homology (>99%) with members of the genus Ochrobactrum in Brucellaceae, although the sequences of other isolates were homologous to those of two distinct genera Bradyrhizobium and Rhizobium. The strain DASA 35030 was strongly suggested to be a strain of Ochrobactrum by full length sequences of the 16S rRNA gene, fatty acids composition, G+C contents of the DNA, and other physiological characteristics. Strain DASA 35030 induced root nodules on A. mangium, A. albida and Paraserianthes falcataria. The nodules formed by strain DASA 35030 fixed nitrogen and the morphology of the nodules is the same as those of nodules formed by the other isolates. This is the first report that the strain of Ochrobactrum possesses complete symbiotic ability with Acacia.

【 授权许可】

Unknown