Analysis of genetic relationships and antimicrobial susceptibility of Escherichia coli isolated from Clethrionomys glareolus

The Journal of General and Applied Microbiology

Agnieszka Iwaniuk¹  Izabela Swiecicka¹  Jan Buczek¹ 

[1] Department of Microbiology, Institute of Biology, University of Bialystok

关键词: antibiotic susceptibility;    Clethrionomys glareolus;    Escherichia coli;    PFGE;    plasmid;   

DOI  :  10.2323/jgam.49.315

学科分类：微生物学和免疫学

来源: Applied Microbiology, Molecular and Cellulrar Biosciences Research Foundation

【 摘 要 】

Eleven strains of Escherichia coli were isolated from 54 bank voles living in the Lomza Landscape Park of the Narew River Valley, indicating that E. coli is not common in the alimentary tract of these mammals. On the basis of pulsed-field gel electrophoresis and computer-assisted analysis, the isolates were grouped into six genotypes at similarities of 39%. Chromosome length of E. coli under study differed by as much as 900kb, ranging 2.7-3.6Mb. All strains were susceptible to amikacin and ciprofloxacin, whereas, for tetracycline, streptomycin, ampicillin, and cefonicid, different results were noted. No differences were detected among the plasmid complements of eight strains (73%), for which plasmid profiles revealed the presence of two plasmidic bands. One, three and four plasmids were observed in a plasmid pattern of single isolates. The observation from the study indicated the high genetic polymorphism among the isolates recovered from the animals of one species living in the same environment.

【 授权许可】

Unknown